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House dust mite extract. A potentially allergenic protein in house dust mites.
A new allergenic protein has been identified in extracts of whole house dust mites. The protein precipitated with ammonium sulphate and appeared to be resistant to the action of proteases. Radioimmunoassay using specific antibodies indicated that mite extract contained about 10(-10) mol of this protein per mg of protein extract. Further fractionation of the extract into different molecular weight fractions resulted in a preparation containing about 10(-10) mol protein/mg. The major protein subunit (Mr 20 kd) was identified in a single band following sodium dodecyl sulphate/polyacrylamide gel electrophoresis. It was specific to house dust mites. The corresponding substance purified from the mite extract was used to raise specific antibodies in rabbits. The antibodies had the specificity of immunoglobulin G and bound the subunit in house dust mites.DPP6-positive cells and more interestingly, by quantification of the percentage of endothelium-derived DPP6-positive cells.
To validate the role of Cx36 in the increased migration and tube formation of RA synovial derived endothelial cells, we used siRNA to knockdown Cx36 expression. We found that the transfection of RA-endothelial cells with siRNA against Cx36 significantly inhibited RA-endothelial cell migration and tube formation. These results suggested that Cx36 is an important factor in the angiogenesis of RA.
Cx36 is a gap junction protein and has been found to be expressed at high levels in the heart, brain, peripheral nerves, retina and pancreas \[[@pone.0118498.ref025]\]. In the heart, Cx
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