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1. The position of several known plasma membrane glycolipids during late stages of chick embryo development was studied in subcellular fractions by staining with specific lectins. 2. The major neutral glycosphingolipids, 3′-sialoganglioside, and 3′-sialylated lactosyl ceramide were found in the plasma membrane and the Golgi apparatus membranes, and increased in amount in the Golgi apparatus membranes as the embryo develops. 3′-Sialylated lactosyl ceramide was also found in the Golgi apparatus membranes of the chick embryo liver, but in much smaller quantities than that of the embryo membrane. 4. Polysialogangliosides were identified only in the plasma membrane and increased in amount in the plasma membrane as the embryo developed. 5. Lactosyl ceramide, a minor glycolipid, was present in similar amounts in all fractions and was not detected after neuraminidase treatment, which eliminated all of the polysialogangliosides. 6. The increased amounts of gangliosides and lactosyl ceramide in the plasma membrane of the embryo were due to an increase in the density of the membrane. Similar increases were also observed in liver and embryo membranes. 7. 3′-Sialylated lactosyl ceramide was also much less sensitive to neuraminidase than the 3′-sialoganglioside and the lactosyl ceramide. 8. The results suggest that the major plasma membrane
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