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Metabolic analysis was undertaken using radioimmunoassays to determine levels of thrombin and factor X in pooled plasma samples obtained from 52 donors undergoing cardiopulmonary bypass (CPB). The conditions of clinical storage affected thrombin and factor X activity differently. When plasma was stored in air at 4 degrees C for 14 days, factor X was markedly more sensitive to inactivation than thrombin. When stored in nitrogen gas, the sensitivity of both proteins was altered, so that thrombin was more sensitive to the duration of storage than factor X. Both the concentrations of factor X and thrombin remained stable in plasma stored at 0 degrees C for 24 h. The CPB technique did not affect the concentration of either thrombin or factor X in plasma. The concentration of factor X was higher in plasma from males than females. The influence of the antiheparin nature of plasma on the thrombin and factor X metabolism was investigated. When purified heparin was used to neutralize the apoprotein, thrombin inactivation was greatly reduced, but factor X activity was not affected by the addition of low concentrations of heparin (0.20 IU/ml), even though high concentrations (3 IU/ml) substantially reduced factor X. The addition of heparin to plasma inhibited thrombin inactivation, but did not affect factor X degradation. A comparison of the properties of ‘crude’ or unfractionated plasma with plasma fractionated into low- and high-molecular-weight fractions suggested a slight degree of protection against the effects of storage and a slowing of thrombin degradation by low-molecular-weight fractions. The development of techniques for the storage of plasma in nitrogen gas may provide a means to prolong the circulation time of cryopreserved plasma.Thermally controlled data storage systems typically use a thermopile which provides a direct measure of the temperature of an
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