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Alveolar macrophages isolated from guinea pigs by bronchoalveolar lavage were used in an in vitro system of cell culture to study the effect of cell death on monocyte generation. Purified macrophages (greater than 95% pure) were incubated in tissue culture plates for up to 48 hr in medium supplemented with heat-inactivated guinea pig serum. After 48 hr, cell viability was determined by trypan blue exclusion, autologous immunofluorescence for surface marker binding, and scanning electron microscopy. The kinetics of generation of a monocyte population was measured by counting viable cells after 24, 48, and 72 hr of incubation in culture. During 48 hr of incubation, the number of viable macrophages decreased by approximately 30% (p less than 0.05). From 24 to 48 hr, macrophage viability decreased by 13.6% (p less than 0.05), 26.6% (p less than 0.05), and 31.1% (p less than 0.05). By 72 hr of incubation, cell viability had decreased by 19.9% (p less than 0.05) and 43.9% (p less than 0.001), compared to 24 and 48 hr, respectively. After 48 hr, conversion to a monocyte population was achieved by 7.35% of the cell population, indicating that virtually all viable macrophages became monocytes. Thus viable macrophages give rise to monocytes at a proportion similar to that observed in vivo.A newly discovered class of
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