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Adenovirus-mediated gene therapy for cancer is limited by low gene transfer efficiency, poor gene expression, and the development of immune responses. The objectives of this study were to evaluate the efficacy of adenovirus-mediated gene therapy in human bladder carcinoma using the E1A protein of Ad5 as a mediator of transgene expression, and to examine the effect of the prior immune response against the Ad5 in a rat bladder carcinoma. Tumors were established by direct injection of UM-3 cells into the bladder wall of rats. When the tumors reached 2 mm in size, animals were divided into the following four groups: control (without treatment), PBS, Ad5, and Ad5 + cytosine deaminase (CD). Gene transfer was administered by intravesicular infusion with Ad5 or Ad5 + CD. Animals were killed 7 days after treatment, and tumor samples were evaluated by immunohistochemistry for CD and the detection of apoptotic cells by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL) assay

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