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Hello, Below Link Is Full Version And All Products Click Here, Alcpt Form 80 Test [FULL Version] Download.zip 1: Alcpt Form 80 Test .Expression and distribution of transcripts for the Bax and Bcl-2 proteins during early preimplantation mouse development.
Bax and Bcl-2 proteins play a pivotal role in determining the survival or death of cells in response to apoptotic stimuli. However, their relative roles in regulating the developmental process during early preimplantation stages have not been well characterized. Thus, we analyzed the distribution and expression of mRNAs for these genes in mouse embryos during stages 1-5 of preimplantation development. Using a reverse transcription-polymerase chain reaction (RT-PCR) technique and in situ hybridization, we determined that the distribution of transcripts of both genes was complementary. For example, Bax mRNA was present in nuclei of all cells at stage 1 of development, whereas Bcl-2 mRNA was detected in all nuclei except for a subpopulation of nuclei at stages 3-5. Furthermore, Bax and Bcl-2 mRNA was located in distinct regions in the cytoplasm. To further investigate the expression of these genes during preimplantation development, we examined the relative levels of their transcripts by quantitative RT-PCR. Using the expression level of GAPDH mRNA as an internal control, relative expression levels of Bax and Bcl-2 mRNAs were determined in the different stages of preimplantation development. As expected, Bax expression was induced at the blastocyst stage compared to the zygote stage. Interestingly, Bcl-2 mRNA levels, on the other hand, were higher at the morula stage compared to the zygote stage. These results indicate that the expression of Bax and Bcl-2 genes might be regulated by distinct mechanisms during preimplantation development.SAGGING.
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